1FMJ

CRYSTAL STRUCTURE OF MERCURY DERIVATIVE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP

Summary for 1FMJ

Entry DOI	10.2210/pdb1fmj/pdb
Related	1FML
Descriptor	RETINOL DEHYDRATASE, MERCURY (II) ION, CALCIUM ION, ... (6 entities in total)
Functional Keywords	sulfotransferase, dehydratase, retinol, adenosine 3', 5'-diphosphate, transferase
Biological source	Spodoptera frugiperda (fall armyworm)
Total number of polymer chains	2
Total formula weight	85821.96
Authors	Pakhomova, S.,Kobayashi, M.,Buck, J.,Newcomer, M.E. (deposition date: 2000-08-17, release date: 2001-05-02, Last modification date: 2024-04-03)
Primary citation	Pakhomova, S.,Kobayashi, M.,Buck, J.,Newcomer, M.E. A helical lid converts a sulfotransferase to a dehydratase. Nat.Struct.Biol., 8:447-451, 2001 Cited by PubMed Abstract: We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase. PubMed: 11323722 DOI: 10.1038/87617 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2 Å)

Structure validation