1FML
CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-14 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.053, 66.612, 84.904 |
Unit cell angles | 90.00, 111.29, 90.00 |
Refinement procedure
Resolution | 38.450 - 2.750 |
Rwork | 0.222 |
R-free | 0.27300 |
Structure solution method | SIRAS Phasing |
RMSD bond length | 0.008 |
RMSD bond angle | 21.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.104 * | 0.371 * |
Number of reflections | 20388 | |
<I/σ(I)> | 12.7 | 3.2 |
Completeness [%] | 92.6 | 98.9 |
Redundancy | 3.2 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 284 | Pakhomova, S., (2000) Acta Crystallogr.D56, 1641. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG3350 | 8 (%) | |
3 | 1 | reservoir | 50 (mM) | ||
4 | 1 | reservoir | HEPES | 100 (mM) |