1FJL

HOMEODOMAIN FROM THE DROSOPHILA PAIRED PROTEIN BOUND TO A DNA OLIGONUCLEOTIDE

1FJL の概要

• エントリーDOI: 10.2210/pdb1fjl/pdb
• 分子名称: PAIRED PROTEIN, DNA (5'-D(*AP*AP*TP*AP*AP*TP*CP*TP*GP*AP*TP*TP*AP*C)-3'), DNA (5'-D(*TP*GP*TP*AP*AP*TP*CP*AP*GP*AP*TP*TP*AP*T)-3'), ... (5 entities in total)
• 機能のキーワード: dna-binding protein, dna, paired box, transcription regulation, transcription-dna complex, transcription/dna
• 由来する生物種: Drosophila melanogaster (fruit fly)
• 細胞内の位置: Nucleus: P06601
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 41976.79

構造登録者

Wilson, D.S.,Guenther, B.,Desplan, C.,Kuriyan, J. (登録日: 1995-12-17, 公開日: 1996-06-20, 最終更新日: 2024-02-07)

主引用文献

Wilson, D.S.,Guenther, B.,Desplan, C.,Kuriyan, J.
High resolution crystal structure of a paired (Pax) class cooperative homeodomain dimer on DNA.
Cell(Cambridge,Mass.), 82:709-719, 1995

PubMed Abstract: The crystal structure of the paired homeodomain bound to DNA as a cooperative dimer has been determined to 2.0 A resolution. Direct contacts between each homeodomain and the DNA are similar to those described previously. In addition, an extensive network of water molecules mediates contacts between the recognition helix and the DNA major groove. Several symmetrical contacts between the two homeodomains underlie the cooperative interaction, and deformations in the DNA structure are necessary for the establishment of these contacts. Comparison with structures of homeodomains bound monomerically to DNA suggests that the binding of a single paired homeodomain can introduce these DNA distortions, thus preparing a template for the cooperative interaction with a second homeodomain. This study shows how the paired (Pax) class homeodomains have achieved cooperativity in DNA binding without the assistance of other domains, thereby enabling the recognition of target sequences that are long enough to ensure specificity.

PubMed: 7671301
DOI: 10.1016/0092-8674(95)90468-9

実験手法

X-RAY DIFFRACTION (2 Å)