1FIU

TETRAMERIC RESTRICTION ENDONUCLEASE NGOMIV IN COMPLEX WITH CLEAVED DNA

1FIU の概要

• エントリーDOI: 10.2210/pdb1fiu/pdb
• 分子名称: DNA (5'-D(*TP*GP*CP*G)-3'), DNA (5'-D(P*CP*CP*GP*GP*CP*GP*C)-3'), TYPE II RESTRICTION ENZYME NGOMI, ... (6 entities in total)
• 機能のキーワード: protein-dna complex, double helix, restriction endonuclease, restriction-modifiction systems, hydrolase, phosphodiesterase, metal ion, complex (endonuclease-dna), hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Neisseria gonorrhoeae
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 140903.12

構造登録者

Deibert, M.,Grazulis, S.,Sasnauskas, G.,Siksnys, V.,Huber, R. (登録日: 2000-08-07, 公開日: 2001-02-07, 最終更新日: 2024-02-07)

主引用文献

Deibert, M.,Grazulis, S.,Sasnauskas, G.,Siksnys, V.,Huber, R.
Structure of the tetrameric restriction endonuclease NgoMIV in complex with cleaved DNA.
Nat.Struct.Biol., 7:792-799, 2000

PubMed Abstract: The crystal structure of the NgoMIV restriction endonuclease in complex with cleaved DNA has been determined at 1.6 A resolution. The crystallographic asymmetric unit contains a protein tetramer and two DNA molecules cleaved at their recognition sites. This is the first structure of a tetrameric restriction enzyme-DNA complex. In the tetramer, two primary dimers are arranged back to back with two oligonucleotides bound in clefts on opposite sides of the tetramer. The DNA molecules retain a B-type conformation and have an enclosed angle between their helical axes of 60 degrees. Sequence-specific interactions occur in both the major and minor grooves. Two Mg2+ ions are located close to the cleaved phosphate at the active site of NgoMIV. Biochemical experiments show that interactions between the recognition sites within the tetramer greatly increase DNA cleavage efficiency.

PubMed: 10966652
DOI: 10.1038/79032

実験手法

X-RAY DIFFRACTION (1.6 Å)