1FI8

RAT GRANZYME B [N66Q] COMPLEXED TO ECOTIN [81-84 IEPD]

1FI8 の概要

• エントリーDOI: 10.2210/pdb1fi8/pdb
• 分子名称: NATURAL KILLER CELL PROTEASE 1, ECOTIN, ... (4 entities in total)
• 機能のキーワード: complex (serine protease-inhibitor), protease substrate interactions, beta strand structure, chymotrypsin fold, granzyme b, ecotin, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• 細胞内の位置: Periplasm: P23827 P23827
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 82799.75

構造登録者

Waugh, S.M.,Harris, J.L.,Fletterick, R.J.,Craik, C.S. (登録日: 2000-08-03, 公開日: 2000-09-13, 最終更新日: 2024-10-16)

主引用文献

Waugh, S.M.,Harris, J.L.,Fletterick, R.,Craik, C.S.
The structure of the pro-apoptotic protease granzyme B reveals the molecular determinants of its specificity
Nat.Struct.Biol., 7:762-765, 2000

PubMed Abstract: Granzyme B is a serine protease of the chymotrypsin fold that mediates cell death by cytotoxic lymphocytes. It is a processing enzyme, requiring extended peptide substrates containing an Asp residue. The determinants that allow for this substrate specificity are revealed in the three-dimensional structure of granzyme B in complex with a macromolecular inhibitor. The primary specificity for Asp occurs through a side-on interaction with Arg 226, a buried Arg side chain of granzyme B. An additional nine amino acids make contact with the substrate and define the granzyme B extended substrate specificity profile. The substrate determinants found in this structure are shared by other members of this protein class and help to reveal the properties that define substrate specificity.

PubMed: 10966646
DOI: 10.1038/78992

実験手法

X-RAY DIFFRACTION (2.2 Å)