1FBA

THE CRYSTAL STRUCTURE OF FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE FROM DROSOPHILA MELANOGASTER AT 2.5 ANGSTROMS RESOLUTION

1FBA の概要

• エントリーDOI: 10.2210/pdb1fba/pdb
• 分子名称: FRUCTOSE 1,6-BISPHOSPHATE ALDOLASE (2 entities in total)
• 機能のキーワード: lyase(aldehyde)
• 由来する生物種: Drosophila melanogaster (fruit fly)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 155956.72

構造登録者

Piontek, K.,Hester, G.,Brenner-Holzach, O. (登録日: 1992-06-08, 公開日: 1993-10-31, 最終更新日: 2024-10-16)

主引用文献

Hester, G.,Brenner-Holzach, O.,Rossi, F.A.,Struck-Donatz, M.,Winterhalter, K.H.,Smit, J.D.,Piontek, K.
The crystal structure of fructose-1,6-bisphosphate aldolase from Drosophila melanogaster at 2.5 A resolution.
FEBS Lett., 292:237-242, 1991

PubMed Abstract: The structure of fructose-1,6-bisphosphate aldolase from Drosophila melanogaster has been determined by X-ray diffraction at 2.5 A resolution. The insect enzyme crystallizes in space group P2(1)2(1)2(1) with lattice replacement with rabbit muscle aldolase as a search model has been employed to solve the structure. To improve the initial phases real space averaging, including phase extension from 4.0 to 2.5 A, has been applied. Refinement of the atomic positions by molecular dynamics resulted in a crystallographic R-factor of 0.214. The tertiary structure resembles in most parts that of the vertebrate aldolase from rabbit muscle. Significant differences were found in surface loops and the N- and C-terminal regions of the protein. Here we present the first aldolase structure where the functionally important C-terminal arm is described completely.

PubMed: 1959612
DOI: 10.1016/0014-5793(91)80875-4

実験手法

X-RAY DIFFRACTION (1.9 Å)