1F7Y
THE CRYSTAL STRUCTURE OF TWO UUCG LOOPS HIGHLIGHTS THE ROLE PLAYED BY 2'-HYDROXYL GROUPS IN ITS UNUSUAL STABILITY
Summary for 1F7Y
| Entry DOI | 10.2210/pdb1f7y/pdb |
| Descriptor | 16S RIBOSOMAL RNA FRAGMENT, 30S RIBOSOMAL PROTEIN S15, MAGNESIUM ION, ... (6 entities in total) |
| Functional Keywords | uucg, tetraloop, rna, ribosome |
| Biological source | Thermus thermophilus More |
| Total number of polymer chains | 2 |
| Total formula weight | 29612.93 |
| Authors | Ennifar, E.,Nikouline, A.,Serganov, A.,Tishchenko, S.,Nevskaya, N.,Garber, M.,Ehresmann, B.,Ehresmann, C.,Nikonov, S.,Dumas, P. (deposition date: 2000-06-28, release date: 2000-11-22, Last modification date: 2024-10-30) |
| Primary citation | Ennifar, E.,Nikulin, A.,Tishchenko, S.,Serganov, A.,Nevskaya, N.,Garber, M.,Ehresmann, B.,Ehresmann, C.,Nikonov, S.,Dumas, P. The crystal structure of UUCG tetraloop. J.Mol.Biol., 304:35-42, 2000 Cited by PubMed Abstract: All large structured RNAs contain hairpin motifs made of a stem closed by several looped nucleotides. The most frequent loop motif is the UUCG one. This motif belongs to the tetraloop family and has the peculiarity of being highly thermodynamically stable. Here, we report the first crystal structure of two UUCG tetraloops embedded in a larger RNA-protein complex solved at 2.8 A resolution. The two loops present in the asymmetric unit are in a different crystal packing environment but, nevertheless, have an identical conformation. The observed structure is globally close to that obtained in solution by nuclear magnetic resonance. However, subtle differences point to a more detailed picture of the role played by 2'-hydroxyl groups in stabilising this tetraloop. PubMed: 11071808DOI: 10.1006/jmbi.2000.4204 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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