1F7Y

THE CRYSTAL STRUCTURE OF TWO UUCG LOOPS HIGHLIGHTS THE ROLE PLAYED BY 2'-HYDROXYL GROUPS IN ITS UNUSUAL STABILITY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-18
Detector	MARRESEARCH
Spacegroup name	P 64 2 2
Unit cell lengths	128.800, 128.800, 65.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.222
Rwork	0.222
R-free	0.28900
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.038	0.209
Number of reflections	14823 *
Completeness [%]	99.9	99.5
Redundancy	8.1	6.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	20 *	1 M (NH4)2SO4, 100 MM SODIUM CACODYLATE, 30 MM KCL, 1.5 MM MGCL2, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SODIUM CACODYLATE
2	1	1	KCL
3	1	1	MGCL2
4	1	1	(NH4)2SO4
5	1	2	KCL
6	1	2	MGCL2
7	1	2	(NH4)2SO4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNA	6 (mg/ml)
2	1	drop	S15	3 (mg/ml)
3	1	reservoir	ammonium sulfate	1.0 (M)
4	1	reservoir		1.5 (mM)
5	1	reservoir		50 (mM)
6	1	reservoir	sodium cacodylate	50 (mM)	pH6.2