1F4W
CRYSTAL STRUCTURE OF AN ANTI-CARBOHYDRATE ANTIBODY DIRECTED AGAINST VIBRIO CHOLERAE O1 IN COMPLEX WITH ANTIGEN
1F4W の概要
エントリーDOI | 10.2210/pdb1f4w/pdb |
関連するPDBエントリー | 1F4X 1F4Y |
分子名称 | ANTIBODY S-20-4, FAB FRAGMENT, LIGHT CHAIN, ANTIBODY S-20-4, FAB FRAGMENT, HEAVY CHAIN (3 entities in total) |
機能のキーワード | anti-carbohydrate antibody, immune system |
由来する生物種 | Mus musculus (house mouse) 詳細 |
タンパク質・核酸の鎖数 | 2 |
化学式量合計 | 45787.02 |
構造登録者 | |
主引用文献 | Villeneuve, S.,Souchon, H.,Riottot, M.M.,Mazie, J.C.,Lei, P.,Glaudemans, C.P.,Kovac, P.,Fournier, J.M.,Alzari, P.M. Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: molecular basis for serotype specificity. Proc.Natl.Acad.Sci.USA, 97:8433-8438, 2000 Cited by PubMed Abstract: The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is shown to be the primary antigenic determinant. Additional perosamine residues protrude outwards from the Ab surface and contribute only marginally to the binding affinity and specificity. A complementary water-excluding hydrophobic interface and five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The structure reported here explains the serotype specificity of anti-Ogawa Abs and provides a rational basis toward the development of a synthetic carbohydrate-based anti-cholera vaccine. PubMed: 10880560DOI: 10.1073/pnas.060022997 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.3 Å) |
構造検証レポート
検証レポート(詳細版)をダウンロード