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1F4W

CRYSTAL STRUCTURE OF AN ANTI-CARBOHYDRATE ANTIBODY DIRECTED AGAINST VIBRIO CHOLERAE O1 IN COMPLEX WITH ANTIGEN

1F4W の概要
エントリーDOI10.2210/pdb1f4w/pdb
関連するPDBエントリー1F4X 1F4Y
分子名称ANTIBODY S-20-4, FAB FRAGMENT, LIGHT CHAIN, ANTIBODY S-20-4, FAB FRAGMENT, HEAVY CHAIN (3 entities in total)
機能のキーワードanti-carbohydrate antibody, immune system
由来する生物種Mus musculus (house mouse)
詳細
タンパク質・核酸の鎖数2
化学式量合計45787.02
構造登録者
Alzari, P.M.,Souchon, H. (登録日: 2000-06-10, 公開日: 2000-08-02, 最終更新日: 2024-10-30)
主引用文献Villeneuve, S.,Souchon, H.,Riottot, M.M.,Mazie, J.C.,Lei, P.,Glaudemans, C.P.,Kovac, P.,Fournier, J.M.,Alzari, P.M.
Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: molecular basis for serotype specificity.
Proc.Natl.Acad.Sci.USA, 97:8433-8438, 2000
Cited by
PubMed Abstract: The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is shown to be the primary antigenic determinant. Additional perosamine residues protrude outwards from the Ab surface and contribute only marginally to the binding affinity and specificity. A complementary water-excluding hydrophobic interface and five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The structure reported here explains the serotype specificity of anti-Ogawa Abs and provides a rational basis toward the development of a synthetic carbohydrate-based anti-cholera vaccine.
PubMed: 10880560
DOI: 10.1073/pnas.060022997
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.3 Å)
構造検証レポート
Validation report summary of 1f4w
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-20に公開中

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