1EWF

THE 1.7 ANGSTROM CRYSTAL STRUCTURE OF BPI

1EWF の概要

• エントリーDOI: 10.2210/pdb1ewf/pdb
• 関連するPDBエントリー: 1BP1
• 分子名称: BACTERICIDAL/PERMEABILITY-INCREASING PROTEIN, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE (3 entities in total)
• 機能のキーワード: bactericidal, permeability-increasing, lipid-binding, lipopolysaccharide-binding, antibiotic
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted : P17213
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52283.92

構造登録者

Kleiger, G.,Beamer, L.J.,Grothe, R.,Mallick, P.,Eisenberg, D. (登録日: 2000-04-25, 公開日: 2000-06-21, 最終更新日: 2024-10-30)

主引用文献

Kleiger, G.,Beamer, L.J.,Grothe, R.,Mallick, P.,Eisenberg, D.
The 1.7 A crystal structure of BPI: a study of how two dissimilar amino acid sequences can adopt the same fold.
J.Mol.Biol., 299:1019-1034, 2000

PubMed Abstract: We have extended the resolution of the crystal structure of human bactericidal/permeability-increasing protein (BPI) to 1.7 A. BPI has two domains with the same fold, but with little sequence similarity. To understand the similarity in structure of the two domains, we compare the corresponding residue positions in the two domains by the method of 3D-1D profiles. A 3D-1D profile is a string formed by assigning each position in the 3D structure to one of 18 environment classes. The environment classes are defined by the local secondary structure, the area of the residue which is buried from solvent, and the fraction of the area buried by polar atoms. A structural alignment between the two BPI domains was used to compare the 3D-1D environments of structurally equivalent positions. Greater than 31% of the aligned positions have conserved 3D-1D environments, but only 13% have conserved residue identities. Analysis of the 3D-1D environmentally conserved positions helps to identify pairs of residues likely to be important in conserving the fold, regardless of the residue similarity. We find examples of 3D-1D environmentally conserved positions with dissimilar residues which nevertheless play similar structural roles. To generalize our findings, we analyzed four other proteins with similar structures yet dissimilar sequences. Together, these examples show that aligned pairs of dissimilar residues often share similar structural roles, stabilizing dissimilar sequences in the same fold.

PubMed: 10843855
DOI: 10.1006/jmbi.2000.3805

実験手法

X-RAY DIFFRACTION (1.7 Å)