1EWF
THE 1.7 ANGSTROM CRYSTAL STRUCTURE OF BPI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 1998-02-06 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 184.320, 31.230, 80.660 |
Unit cell angles | 90.00, 103.20, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
Rwork | 0.198 |
R-free | 0.25000 |
RMSD bond length | 0.017 |
RMSD bond angle | 25.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.048 | 0.201 |
Number of reflections | 47198 | |
<I/σ(I)> | 16.7 | |
Completeness [%] | 94.2 | 67 |
Redundancy | 2.3 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 289 | 12 % PEG 6000, 0.2 M Mg Acetate, 0.1 M Na Cacodylate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 16K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.5 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 12 (%(w/v)) | |
3 | 1 | reservoir | magnesium acetate | 200 (mM) | |
4 | 1 | reservoir | sodium cacodylate | 100 (mM) |