1EVN
NMR OBSERVATION OF A-TETRAD
Summary for 1EVN
| Entry DOI | 10.2210/pdb1evn/pdb |
| Descriptor | DNA (5'-D(*AP*GP*GP*GP*T)-3') (1 entity in total) |
| Functional Keywords | a-tetrad, human telomere, g-quadruplex, dna |
| Total number of polymer chains | 4 |
| Total formula weight | 6240.24 |
| Authors | Patel, P.K.,Koti, A.S.R.,Hosur, R.V. (deposition date: 2000-04-20, release date: 2000-05-22, Last modification date: 2024-05-22) |
| Primary citation | Patel, P.K.,Koti, A.S.,Hosur, R.V. NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad. Nucleic Acids Res., 27:3836-3843, 1999 Cited by PubMed Abstract: The structure of the telomeric DNA has been a subject of extensive investigation in recent years due to the realization that it has important functional roles to play in vivo and the observations that truncated telomeric sequences exhibit a great variety of 3D structures in aqueous solutions. In this context, we describe here NMR structural studies on two truncated human telomeric DNA sequences, d-AG(3)T and d-TAG(3)T in solutions containing K(+)ions. The G(3)stretches in both the oligonucleotides were seen to form parallel-stranded quadruplexes. However, the AG(3)segment as a whole, had different structural characteristics. The structure of d-AG(3)T revealed the formation of a novel A-tetrad, which was not seen in d-TAG(3)T. The A's in the tetrad had syn glycosidic conformation as opposed to the anti conformation of the G's in the G-tetrads. The A-tetrad stacked well over the adjacent G-tetrad and the twist angle at this step was smaller in d-AG(3)T than in d-TAG(3)T. These observations are expected to be significant from the point of view of structural diversity and recognition in telomeres. PubMed: 10481022DOI: 10.1093/nar/27.19.3836 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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