1EQP

EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS

1EQP の概要

• エントリーDOI: 10.2210/pdb1eqp/pdb
• 関連するPDBエントリー: 1CZ1 1EQC
• 分子名称: EXO-B-(1,3)-GLUCANASE (2 entities in total)
• 機能のキーワード: candida albicans, exoglucanase, alternative codon usage, hydrolase
• 由来する生物種: Candida albicans
• 細胞内の位置: Secreted, cell wall : P29717
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45243.80

構造登録者

Cutfield, J.F.,Sullivan, P.A.,Cutfield, S.M. (登録日: 2000-04-05, 公開日: 2001-02-28, 最終更新日: 2024-10-30)

主引用文献

Cutfield, J.F.,Sullivan, P.A.,Cutfield, S.M.
Minor structural consequences of alternative CUG codon usage (Ser for Leu) in Candida albicans exoglucanase.
Protein Eng., 13:735-738, 2000

PubMed Abstract: In some species of Candida the CUG codon is encoded as serine and not leucine. In the case of the exo-beta-1,3-glucanase from the pathogenic fungus C. albicans there are two such translational events, one in the prepro-leader sequence and the other at residue 64. Overexpression of active mature enzyme in a yeast host indicated that these two positions are tolerant to substitution. By comparing the crystal structure of the recombinant protein with that of the native (presented here), it is seen how either serine or leucine can be accommodated at position 64. Examination of the relatively few solved protein structures from C. albicans indicates that other CUG encoded serines are also found at non-essential surface sites. However such codon usage is rare in C. albicans, in contrast to C. rugosa, with direct implications for respective recombinant protein production.

PubMed: 11112513
DOI: 10.1093/protein/13.10.735

実験手法

X-RAY DIFFRACTION (1.9 Å)