1EJB

LUMAZINE SYNTHASE FROM SACCHAROMYCES CEREVISIAE

1EJB の概要

• エントリーDOI: 10.2210/pdb1ejb/pdb
• 関連するPDBエントリー: 1C2Y 1C41 1DI0 1RVV
• 分子名称: LUMAZINE SYNTHASE, 5-(6-D-RIBITYLAMINO-2,4-DIHYDROXYPYRIMIDIN-5-YL)-1-PENTYL-PHOSPHONIC ACID (3 entities in total)
• 機能のキーワード: lumazine synthase, saccharomyces cerevisiae, x-ray structure analysis, inhibitor complex, vitamin biosynthesis, transferase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Mitochondrion intermembrane space : P50861
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 94298.30

構造登録者

Meining, W.,Mortl, S.,Fischer, M.,Cushman, M.,Bacher, A.,Ladenstein, R. (登録日: 2000-03-02, 公開日: 2001-03-02, 最終更新日: 2024-02-07)

主引用文献

Meining, W.,Mortl, S.,Fischer, M.,Cushman, M.,Bacher, A.,Ladenstein, R.
The atomic structure of pentameric lumazine synthase from Saccharomyces cerevisiae at 1.85 A resolution reveals the binding mode of a phosphonate intermediate analogue.
J.Mol.Biol., 299:181-197, 2000

PubMed Abstract: Lumazine synthase of Saccharomyces cerevisiae is a homopentamer with a molecular weight of 90 kDa. Crystals of the recombinant enzyme with a size of up to 1.6 mm were obtained. The space group is P4(1)2(1)2 with lattice dimensions 82.9 A x 82.9 A x 300.2 A. X-ray diffraction data collected under cryogenic conditions were complete to 1.85 A resolution. The structure of the enzyme in complex with the intermediate analogue, 5-(6-D-ribitylamino-2,4-dihydroxypyrimidine-5-yl)-1-pentyl-p hosphonic acid was solved via molecular replacement using the structure of the Bacillus subtilis enzyme as search model and was refined to a final R-factor of 19.8% (Rfree: 22.5%). The conformation of the active site ligand of the enzyme mimicks that of the Schiff base intermediate of the enzyme-catalyzed reaction. The data enable the reconstruction of the reactant topology during the early steps of the catalytic reaction. Structural determinants, which are likely to be responsible for the inability of the S. cerevisiae enzyme to form icosahedral capsids, will be discussed.

PubMed: 10860731
DOI: 10.1006/jmbi.2000.3742

実験手法

X-RAY DIFFRACTION (1.85 Å)