1EHV

A NEW CRYSTAL STRUCTURE FOR THE DODECAMER C-G-C-G-A-A-T-T-C-G-C-G: SYMMETRY EFFECTS ON SEQUENCE-DEPENDENT DNA STRUCTURE

Summary for 1EHV

• Entry DOI: 10.2210/pdb1ehv/pdb
• Descriptor: DNA (5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3') (2 entities in total)
• Functional Keywords: dickerson dodecamer, crystal packing, dna
• Total number of polymer chains: 1
• Total formula weight: 3663.39

Authors

Johansson, E.M. (deposition date: 2000-02-23, release date: 2000-07-14, Last modification date: 2024-02-07)

Primary citation

Johansson, E.,Parkinson, G.,Neidle, S.
A new crystal form for the dodecamer C-G-C-G-A-A-T-T-C-G-C-G: symmetry effects on sequence-dependent DNA structure.
J.Mol.Biol., 300:551-561, 2000

PubMed Abstract: The dodecanucleotide d(CGCGAATTCGCG) has been crystallised in the space group P3(2)12, representing a new crystal form for this sequence. The structure has been solved by molecular replacement and refined at 1.8 A resolution. The present structure contrasts with previous ones for this sequence since it is situated on a crystallographic 2-fold axis, and the crystal symmetry reflects the palindromic nature of this sequence. Some features accord with previous observations, notably that the minor groove is hydrated with a continuous spine of solvent. There is no evidence of alkali metal ions within this spine. The minor groove retains its narrow width, although it is now symmetric and extends over the A/T tract. Various base and base-pair morphological parameters have been examined. Their values do not show significant correlations with earlier reports, suggesting that crystal packing effects on them are more dominant than has been hitherto realised.

PubMed: 10884351
DOI: 10.1006/jmbi.2000.3907

Experimental method

X-RAY DIFFRACTION (1.8 Å)