1EHK

CRYSTAL STRUCTURE OF THE ABERRANT BA3-CYTOCHROME-C OXIDASE FROM THERMUS THERMOPHILUS

Summary for 1EHK

• Entry DOI: 10.2210/pdb1ehk/pdb
• Descriptor: BA3-TYPE CYTOCHROME-C OXIDASE, nonyl beta-D-glucopyranoside, COPPER (II) ION, ... (9 entities in total)
• Functional Keywords: cytochrome-c oxidase, membrane protein, thermus thermophilus, oxidoreductase
• Biological source: Thermus thermophilus; More
• Total number of polymer chains: 3
• Total formula weight: 87440.07

Authors

Soulimane, T.,Buse, G.,Bourenkov, G.P.,Bartunik, H.D.,Huber, R.,Than, M.E. (deposition date: 2000-02-21, release date: 2001-02-22, Last modification date: 2024-11-13)

Primary citation

Soulimane, T.,Buse, G.,Bourenkov, G.P.,Bartunik, H.D.,Huber, R.,Than, M.E.
Structure and mechanism of the aberrant ba(3)-cytochrome c oxidase from thermus thermophilus.
EMBO J., 19:1766-1776, 2000

PubMed Abstract: Cytochrome c oxidase is a respiratory enzyme catalysing the energy-conserving reduction of molecular oxygen to water. The crystal structure of the ba(3)-cytochrome c oxidase from Thermus thermophilus has been determined to 2.4 A resolution using multiple anomalous dispersion (MAD) phasing and led to the discovery of a novel subunit IIa. A structure-based sequence alignment of this phylogenetically very distant oxidase with the other structurally known cytochrome oxidases leads to the identification of sequence motifs and residues that seem to be indispensable for the function of the haem copper oxidases, e.g. a new electron transfer pathway leading directly from Cu(A) to Cu(B). Specific features of the ba(3)-oxidase include an extended oxygen input channel, which leads directly to the active site, the presence of only one oxygen atom (O(2-), OH(-) or H(2)O) as bridging ligand at the active site and the mainly hydrophobic character of the interactions that stabilize the electron transfer complex between this oxidase and its substrate cytochrome c. New aspects of the proton pumping mechanism could be identified.

PubMed: 10775261
DOI: 10.1093/emboj/19.8.1766

Experimental method

X-RAY DIFFRACTION (2.4 Å)