1E7K

Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment

Summary for 1E7K

• Entry DOI: 10.2210/pdb1e7k/pdb
• Descriptor: 15.5 KD RNA BINDING PROTEIN, RNA (5'-R(*GP*CP*CP*AP*AP*UP*GP*AP*GP*GP*UP*UP*UP* AP*UP*CP*CP*GP*AP*GP*G*C(-3') (2 entities in total)
• Functional Keywords: rna-binding protein, splicing, rna recognition motif, u4 snrna, rna binding protein
• Biological source: HOMO SAPIENS (HUMAN); More
• Total number of polymer chains: 4
• Total formula weight: 42531.55

Authors

Vidovic, I.,Nottrott, S.,Harthmuth, K.,Luhrmann, R.,Ficner, R. (deposition date: 2000-08-29, release date: 2001-02-19, Last modification date: 2024-05-08)

Primary citation

Vidovic, I.,Nottrott, S.,Hartmuth, K.,Luhrmann, R.,Ficner, R.
Crystal Structure of the Spliceosomal 15.5Kd Protein Bound to a U4 Snrna Fragment
Mol.Cell, 6:1331-, 2000

PubMed Abstract: We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins.

PubMed: 11163207
DOI: 10.1016/S1097-2765(00)00131-3

Experimental method

X-RAY DIFFRACTION (2.9 Å)