1E50

AML1/CBFbeta complex

1E50 の概要

• エントリーDOI: 10.2210/pdb1e50/pdb
• 分子名称: CORE-BINDING FACTOR ALPHA SUBUNIT, CORE-BINDING FACTOR CBF-BETA (3 entities in total)
• 機能のキーワード: transcription factor, transcription
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Nucleus: Q01196 Q13951
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 152717.52

構造登録者

Warren, A.J.,Bravo, J.,Williams, R.L.,Rabbits, T.H. (登録日: 2000-07-13, 公開日: 2001-07-12, 最終更新日: 2024-05-08)

主引用文献

Warren, A.J.,Bravo, J.,Williams, R.L.,Rabbits, T.H.
Structural Basis for the Heterodimeric Interaction between the Acute Leukaemia-Associated Transcription Factors Aml1 and Cbfbeta
Embo J., 19:3004-, 2000

PubMed Abstract: Mutations in the genes encoding the interacting proteins AML1 and CBFbeta are the most common genetic abnormalities in acute leukaemia, and congenital mutations in the related AML3 gene are associated with disorders of osteogenesis. Furthermore, the interaction of AML1 with CBFbeta is essential for haematopoiesis. We report the 2.6 A resolution crystal structure of the complex between the AML1 Runt domain and CBFbeta, which represents a paradigm for the mode of interaction of this highly conserved family of transcription factors. The structure demonstrates that point mutations associated with cleidocranial dysplasia map to the conserved heterodimer interface, suggesting a role for CBFbeta in osteogenesis, and reveals a potential protein interaction platform composed of conserved negatively charged residues on the surface of CBFbeta.

PubMed: 10856244
DOI: 10.1093/EMBOJ/19.12.3004

実験手法

X-RAY DIFFRACTION (2.6 Å)