1E1K

ADRENODOXIN REDUCTASE in complex with NADP+ obtained by a soaking experiment

1E1K の概要

• エントリーDOI: 10.2210/pdb1e1k/pdb
• 関連するPDBエントリー: 1CJC 1E1L 1E1M 1E1N
• 分子名称: ADRENODOXIN REDUCTASE, FLAVIN-ADENINE DINUCLEOTIDE, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (4 entities in total)
• 機能のキーワード: oxidoreductase, nadp+, flavoenzyme, electron transferase
• 由来する生物種: BOVINE
• 細胞内の位置: Mitochondrion matrix: P08165
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 51889.37

構造登録者

Ziegler, G.A.,Schulz, G.E. (登録日: 2000-05-09, 公開日: 2000-09-24, 最終更新日: 2023-12-06)

主引用文献

Ziegler, G.A.,Schulz, G.E.
Crystal Structures of Adrenodoxin Reductase in Complex with Nadp+ and Nadph Suggesting a Mechanism for the Electron Transfer of an Enzyme Family
Biochemistry, 39:10986-, 2000

PubMed Abstract: Adrenodoxin reductase is a flavoenzyme that shuffles electrons for the biosynthesis of steroids. Its chain topology belongs to the glutathione reductase family of disulfide oxidoreductases, all of which bind FAD at equivalent positions. The three reported structures of adrenodoxin reductase were ligated with reduced and oxidized NADP and have now confirmed this equivalence also for the NADP-binding site. Remarkably, the conformations and relative positions of the prosthetic group FAD and the cofactor NADP have been conserved during protein evolution despite very substantial changes in the polypeptide. The ligated enzymes showed small changes in the domain positions. When compared with the structure of the NADP-free enzyme, these positions correspond to several states of the domain motion during NADP binding. On the basis of the observed structures, we suggest an enzymatic mechanism for the subdivision of the received two-electron package into the two single electrons transferred to the carrier protein adrenodoxin. The data banks contain 10 sequences that are closely related to bovine adrenodoxin reductase. Most of them code for gene products with unknown functions. Within this family, the crucial residues of adrenodoxin reductase are strictly conserved. Moreover, the putative docking site of the carrier is rather well conserved. Five of the family members were assigned names related to ferredoxin:NADP(+) reductase, presumably because adrenodoxin reductase was considered a member of this functionally similar family. Since this is not the case, the data bank entries should be corrected.

PubMed: 10998235
DOI: 10.1021/BI000079K

実験手法

X-RAY DIFFRACTION (1.95 Å)