1E1K

ADRENODOXIN REDUCTASE in complex with NADP+ obtained by a soaking experiment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Collection date	1999-07-15
Spacegroup name	P 1 21 1
Unit cell lengths	60.900, 62.400, 78.200
Unit cell angles	90.00, 106.60, 90.00

Refinement procedure

Resolution	29.000 * - 1.950
Rwork	0.184
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cjc
RMSD bond length	0.012
RMSD bond angle	2.400 *
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000 *	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.069 *	0.330 *
Number of reflections	41174
<I/σ(I)>	10	2.3
Completeness [%]	100.0	100
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	20 *	pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop	glycerol	5 (%)
3	1	drop	PEG8000	8 (%(w/v))
4	1	drop	sodium cacodylate	50 (mM)	Buffer C
5	1	drop	calcium acetate	100 (mM)	Buffer C
6	1	reservoir	Buffer C
7	1	reservoir	PEG8000	12 (%(w/v))