1E1K
ADRENODOXIN REDUCTASE in complex with NADP+ obtained by a soaking experiment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Collection date | 1999-07-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.900, 62.400, 78.200 |
| Unit cell angles | 90.00, 106.60, 90.00 |
Refinement procedure
| Resolution | 29.000 * - 1.950 |
| Rwork | 0.184 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cjc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.400 * |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 * | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.069 * | 0.330 * |
| Number of reflections | 41174 | |
| <I/σ(I)> | 10 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 20 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | glycerol | 5 (%) | |
| 3 | 1 | drop | PEG8000 | 8 (%(w/v)) | |
| 4 | 1 | drop | sodium cacodylate | 50 (mM) | Buffer C |
| 5 | 1 | drop | calcium acetate | 100 (mM) | Buffer C |
| 6 | 1 | reservoir | Buffer C | ||
| 7 | 1 | reservoir | PEG8000 | 12 (%(w/v)) |
