1E12

Halorhodopsin, a light-driven chloride pump

1E12 の概要

• エントリーDOI: 10.2210/pdb1e12/pdb
• 関連するPDBエントリー: 1BRR
• 分子名称: HALORHODOPSIN, CHLORIDE ION, POTASSIUM ION, ... (7 entities in total)
• 機能のキーワード: ion pump, chloride pump, membrane protein, retinal protein, lipids, palmitate, haloarchaea, chloride transport, transport protein
• 由来する生物種: HALOBACTERIUM SALINARUM
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31127.43

構造登録者

Essen, L.-O.,Kolbe, M.,Oesterhelt, D. (登録日: 2000-04-14, 公開日: 2000-06-02, 最終更新日: 2024-10-16)

主引用文献

Kolbe, M.,Besir, H.,Essen, L.-O.,Oesterhelt, D.
Structure of Light-Driven Chloride Pump Halorhodopsin at 1.8 A Resolution
Science, 288:1390-, 2000

PubMed Abstract: Halorhodopsin, an archaeal rhodopsin ubiquitous in Haloarchaea, uses light energy to pump chloride through biological membranes. Halorhodopsin crystals were grown in a cubic lipidic phase, which allowed the x-ray structure determination of this anion pump at 1.8 angstrom resolution. Halorhodopsin assembles to trimers around a central patch consisting of palmitic acid. Next to the protonated Schiff base between Lys(242) and the isomerizable retinal chromophore, a single chloride ion occupies the transport site. Energetic calculations on chloride binding reveal a combination of ion-ion and ion-dipole interactions for stabilizing the anion 18 angstroms below the membrane surface. Ion dragging across the protonated Schiff base explains why chloride and proton translocation modes are mechanistically equivalent in archaeal rhodopsins.

PubMed: 10827943
DOI: 10.1126/SCIENCE.288.5470.1390

実験手法

X-RAY DIFFRACTION (1.8 Å)