1E09

Solution Structure of the Major Cherry Allergen Pru av 1

Summary for 1E09

• Entry DOI: 10.2210/pdb1e09/pdb
• Related: 1B6F 1BTV 1BV1
• Descriptor: PRU AV 1 (1 entity in total)
• Functional Keywords: allergen, major cherry allergen, pathogenesis-related protein, heteronuclear nmr
• Biological source: PRUNUS AVIUM (SWEET CHERRY)
• Total number of polymer chains: 1
• Total formula weight: 17553.68

Authors

Neudecker, P.,Nerkamp, J.,Schweimer, K.,Sticht, H.,Boehm, M.,Scheurer, S.,Vieths, S.,Roesch, P. (deposition date: 2000-03-15, release date: 2001-03-15, Last modification date: 2024-05-15)

Primary citation

Neudecker, P.,Schweimer, K.,Nerkamp, J.,Scheurer, S.,Vieths, S.,Sticht, H.,Roesch, P.
Allergic Cross-Reactivity Made Visible: The Solution Structure of the Major Cherry Allergen Pru Av 1
J.Biol.Chem., 276:22756-, 2001

PubMed Abstract: Birch pollinosis is often accompanied by hypersensitivity to fruit as a consequence of the cross-reaction of pollen allergen-specific IgE antibodies with homologous food proteins. To provide a basis for examining the cross-reactivity on a structural level, we used heteronuclear multidimensional NMR spectroscopy to determine the high-resolution three-dimensional structure of the major cherry allergen, Pru av 1, in solution. Based on a detailed comparison of the virtually identical structures of Pru av 1 and Bet v 1, the major birch pollen allergen, we propose an explanation for a significant aspect of the observed cross-reactivity pattern among the family of allergens under consideration. The large hydrophobic cavity expected to be important for the still unknown physiological function of Bet v 1 is conserved in Pru av 1. Structural homology to a domain of human MLN64 associated with cholesterol transport suggests phytosteroids as putative ligands for Pru av 1. NMR spectroscopy provides experimental evidence that Pru av 1 interacts with phytosteroids, and molecular modeling shows that the hydrophobic cavity is large enough to accommodate two such molecules.

PubMed: 11287426
DOI: 10.1074/JBC.M101657200

Experimental method

SOLUTION NMR