1DZL

L1 protein of human papillomavirus 16

Summary for 1DZL

• Entry DOI: 10.2210/pdb1dzl/pdb
• Descriptor: LATE MAJOR CAPSID PROTEIN L1 (1 entity in total)
• Functional Keywords: virus, icosahedral
• Biological source: HUMAN PAPILLOMAVIRUS TYPE 16
• Total number of polymer chains: 1
• Total formula weight: 56441.11

Authors

Chen, X.J.,Garcea, B.,Goldberge, I.,Harrison, S.C. (deposition date: 2000-03-01, release date: 2000-03-09, Last modification date: 2025-02-19)

Primary citation

Chen, X.J.,Garcea, B.,Goldberg, I.,Casini, G.,Harrison, S.C.
Structure of Small Virus-Like Particles Assembled from the L1 Protein of Human Papillomavirus 16
Mol.Cell, 5:557-, 2000

PubMed Abstract: The papillomavirus major late protein, L1, forms the pentameric assembly unit of the viral shell. Recombinant HPV16 L1 pentamers assemble in vitro into capsid-like structures, and truncation of ten N-terminal residues leads to a homogeneous preparation of 12-pentamer, icosahedral particles. X-ray crystallographic analysis of these particles at 3.5 A resolution shows that L1 closely resembles VP1 from polyomaviruses. Surface loops contain the sites of sequence variation among HPV types and the locations of dominant neutralizing epitopes. The ease with which small virus-like particles may be obtained from L1 expressed in E. coli makes them attractive candidate components of a papillomavirus vaccine. Their crystal structure also provides a starting point for future vaccine design.

PubMed: 10882140
DOI: 10.1016/S1097-2765(00)80449-9

Experimental method

X-RAY DIFFRACTION (3.5 Å)