1DZ5

The NMR structure of the 38KDa U1A protein-PIE RNA complex reveals the basis of cooperativity in regulation of polyadenylation by human U1A protein

1DZ5 の概要

• エントリーDOI: 10.2210/pdb1dz5/pdb
• 関連するPDBエントリー: 1AUD 1FHT 1URN
• 分子名称: U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A, PIE, RNA (5'-R(*GP*AP*GP*AP*CP*AP*UP*UP*GP*CP*AP*CP*CP* CP*GP*GP*AP*GP*UP*CP*UP*C)-3') (2 entities in total)
• 機能のキーワード: ribonucleoprotein-rna complex, polyadenylation, protein protein interaction, rna protein interaction, ribonucleoprotein/rna
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Nucleus: P09012
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 37293.65

構造登録者

Varani, L.,Gunderson, S.I.,Mattaj, I.W.,Kay, L.E.,Neuhaus, D.,Varani, G. (登録日: 2000-02-16, 公開日: 2000-03-29, 最終更新日: 2024-05-15)

主引用文献

Varani, L.,Gunderson, S.I.,Mattaj, I.W.,Kay, L.E.,Neuhaus, D.,Varani, G.
The NMR Structure of the 38kDa U1A Protein-Pie RNA Complex Reveals the Basis of Cooperativity in Regulation of Polyadenylation by Human U1A Protein
Nat.Struct.Biol., 7:329-, 2000

PubMed Abstract: The status of the poly(A) tail at the 3'-end of mRNAs controls the expression of numerous genes in response to developmental and extracellular signals. Poly(A) tail regulation requires cooperative binding of two human U1A proteins to an RNA regulatory region called the polyadenylation inhibition element (PIE). When bound to PIE RNA, U1A proteins also bind to the enzyme responsible for formation of the mature 3'-end of most eukaryotic mRNAs, poly(A) polymerase (PAP). The NMR structure of the 38 kDa complex formed between two U1A molecules and PIE RNA shows that binding cooperativity depends on helix C located at the end of the RNA-binding domain and just adjacent to the PAP-interacting domain of U1A. Since helix C undergoes a conformational change upon RNA binding, the structure shows that binding cooperativity and interactions with PAP occur only when U1A is bound to its cognate RNA. This mechanism ensures that the activity of PAP enzyme, which is essential to the cell, is only down regulated when U1A is bound to the U1A mRNA.

PubMed: 10742179
DOI: 10.1038/74101

実験手法

SOLUTION NMR