1DT0

CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS

1DT0 の概要

• エントリーDOI: 10.2210/pdb1dt0/pdb
• 関連するPDBエントリー: 1ar4 1ids 1isb 1isc
• 分子名称: SUPEROXIDE DISMUTASE, FE (III) ION (3 entities in total)
• 機能のキーワード: superoxide dismutase, pseudomonas ovalis, oxidoreductase
• 由来する生物種: Pseudomonas putida
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 65901.92

構造登録者

Bond, C.J.,Huang, J.,Hajduk, R.,Flick, K.,Heath, P.,Stoddard, B.L. (登録日: 2000-01-10, 公開日: 2000-12-20, 最終更新日: 2024-02-07)

主引用文献

Bond, C.J.,Huang, J.,Hajduk, R.,Flick, K.E.,Heath, P.J.,Stoddard, B.L.
Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis.
Acta Crystallogr.,Sect.D, 56:1359-1366, 2000

PubMed Abstract: The gene encoding the iron-dependent superoxide dismutase from Pseudomonas ovalis was cloned from a genomic library and sequenced. The ORF differs from the previously published protein sequence, which was used for the original structure determination, at 16 positions. The differences include three additional inserted residues, one deleted residue and 12 point substitutions. The gene was subcloned and the recombinant protein overexpressed, purified and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 A resolution.

PubMed: 11053832
DOI: 10.1107/S0907444900009537

実験手法

X-RAY DIFFRACTION (2.1 Å)