1DLS

METHOTREXATE-RESISTANT VARIANTS OF HUMAN DIHYDROFOLATE REDUCTASE WITH SUBSTITUTION OF LEUCINE 22: KINETICS, CRYSTALLOGRAPHY AND POTENTIAL AS SELECTABLE MARKERS

Summary for 1DLS

• Entry DOI: 10.2210/pdb1dls/pdb
• Descriptor: DIHYDROFOLATE REDUCTASE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, METHOTREXATE, ... (4 entities in total)
• Functional Keywords: oxido-reductase, oxidoreductase
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 22599.40

Authors

Cody, V.,Galitsky, N.,Luft, J.R.,Pangborn, W. (deposition date: 1995-01-25, release date: 1995-04-20, Last modification date: 2024-02-07)

• Primary citation: Lewis, W.S.,Cody, V.,Galitsky, N.,Luft, J.R.,Pangborn, W.,Chunduru, S.K.,Spencer, H.T.,Appleman, J.R.,Blakley, R.L.; Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers.; J.Biol.Chem., 270:5057-5064, 1995; PubMed: 7890613; DOI: 10.1074/jbc.270.10.5057

Experimental method

X-RAY DIFFRACTION (2.3 Å)