1DLS
METHOTREXATE-RESISTANT VARIANTS OF HUMAN DIHYDROFOLATE REDUCTASE WITH SUBSTITUTION OF LEUCINE 22: KINETICS, CRYSTALLOGRAPHY AND POTENTIAL AS SELECTABLE MARKERS
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | DIHYDROFOLATE REDUCTASE | polymer | 186 | 21399.5 | 1 | UniProt (P00374) Pfam (PF00186) In PDB | Homo sapiens (human) | |
| 2 | A | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 1 | Chemie (NDP) | |||
| 3 | A | METHOTREXATE | non-polymer | 454.4 | 1 | Chemie (MTX) | |||
| 4 | water | water | 18.0 | 111 | Chemie (HOH) |
Sequence modifications
A: 1 - 186 (UniProt: P00374)
| PDB | External Database | Details |
|---|---|---|
| Tyr 22 | Leu 22 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 21399.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1199.9 | |
| All* | Total formula weight | 22599.4 |
