1DLR
METHOTREXATE-RESISTANT VARIANTS OF HUMAN DIHYDROFOLATE REDUCTASE WITH SUBSTITUTION OF LEUCINE 22: KINETICS, CRYSTALLOGRAPHY AND POTENTIAL AS SELECTABLE MARKERS
Summary for 1DLR
Entry DOI | 10.2210/pdb1dlr/pdb |
Descriptor | DIHYDROFOLATE REDUCTASE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, 6-(2,5-DIMETHOXY-BENZYL)-5-METHYL-PYRIDO[2,3-D]PYRIMIDINE-2,4-DIAMINE, ... (4 entities in total) |
Functional Keywords | oxido-reductase, oxidoreductase |
Biological source | Homo sapiens (human) |
Total number of polymer chains | 1 |
Total formula weight | 22454.33 |
Authors | Cody, V.,Galitsky, N.,Luft, J.R.,Pangborn, W. (deposition date: 1995-01-25, release date: 1995-04-20, Last modification date: 2024-02-07) |
Primary citation | Lewis, W.S.,Cody, V.,Galitsky, N.,Luft, J.R.,Pangborn, W.,Chunduru, S.K.,Spencer, H.T.,Appleman, J.R.,Blakley, R.L. Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers. J.Biol.Chem., 270:5057-5064, 1995 Cited by PubMed: 7890613DOI: 10.1074/jbc.270.10.5057 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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