1DFU

CRYSTAL STRUCTURE OF E.COLI RIBOSOMAL PROTEIN L25 COMPLEXED WITH A 5S RRNA FRAGMENT AT 1.8 A RESOLUTION

1DFU の概要

• エントリーDOI: 10.2210/pdb1dfu/pdb
• 分子名称: 5S RRNA, RIBOSOMAL PROTEIN L25, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: protein-rna complex, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 23197.45

構造登録者

Lu, M.,Steitz, T.A. (登録日: 1999-11-21, 公開日: 1999-12-02, 最終更新日: 2024-02-07)

主引用文献

Lu, M.,Steitz, T.A.
Structure of Escherichia coli ribosomal protein L25 complexed with a 5S rRNA fragment at 1.8-A resolution.
Proc.Natl.Acad.Sci.USA, 97:2023-2028, 2000

PubMed Abstract: The crystal structure of Escherichia coli ribosomal protein L25 bound to an 18-base pair portion of 5S ribosomal RNA, which contains "loop E," has been determined at 1.8-A resolution. The protein primarily recognizes a unique RNA shape, although five side chains make direct or water-mediated interactions with bases. Three beta-strands lie in the widened minor groove of loop E formed by noncanonical base pairs and cross-strand purine stacks, and an alpha-helix interacts in an adjacent widened major groove. The structure of loop E is largely the same as that of uncomplexed RNA (rms deviation of 0.4 A for 11 base pairs), and 3 Mg(2+) ions that stabilize the noncanonical base pairs lie in the same or similar locations in both structures. Perhaps surprisingly, those residues interacting with the RNA backbone are the most conserved among known L25 sequences, whereas those interacting with the bases are not.

PubMed: 10696113
DOI: 10.1073/pnas.97.5.2023

実験手法

X-RAY DIFFRACTION (1.8 Å)