1DCH

CRYSTAL STRUCTURE OF DCOH, A BIFUNCTIONAL, PROTEIN-BINDING TRANSCRIPTION COACTIVATOR

1DCH の概要

• エントリーDOI: 10.2210/pdb1dch/pdb
• 分子名称: DCOH (DIMERIZATION COFACTOR OF HNF-1), SULFATE ION (2 entities in total)
• 機能のキーワード: transcriptional simulator, dimerization cofactor, dehydratase, 4a-carbinolamine dehydratase, transregulator of homeodomain proteins, transcriptional stimulator, dimerization
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 96909.33

構造登録者

Endrizzi, J.A.,Cronk, J.D.,Wang, W.,Crabtree, G.R.,Alber, T. (登録日: 1995-01-24, 公開日: 1996-03-08, 最終更新日: 2024-02-07)

主引用文献

Endrizzi, J.A.,Cronk, J.D.,Wang, W.,Crabtree, G.R.,Alber, T.
Crystal structure of DCoH, a bifunctional, protein-binding transcriptional coactivator.
Science, 268:556-559, 1995

PubMed Abstract: DCoH, the dimerization cofactor of hepatocyte nuclear factor-1, stimulates gene expression by associating with specific DNA binding proteins and also catalyzes the dehydration of the biopterin cofactor of phenylalanine hydroxylase. The x-ray crystal structure determined at 3 angstrom resolution reveals that DCoH forms a tetramer containing two saddle-shaped grooves that comprise likely macromolecule binding sites. Two equivalent enzyme active sites flank each saddle, suggesting that there is a spatial connection between the catalytic and binding activities. Structural similarities between the DCoH fold and nucleic acid-binding proteins argue that the saddle motif has evolved to bind diverse ligands or that DCoH unexpectedly may bind nucleic acids.

PubMed: 7725101

実験手法

X-RAY DIFFRACTION (3 Å)