1D8X

CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G A BASE PAIRS

Summary for 1D8X

• Entry DOI: 10.2210/pdb1d8x/pdb
• Descriptor: 5'-D(*CP*CP*GP*AP*AP*TP*GP*AP*GP*G)-3', MAGNESIUM ION, COBALT HEXAMMINE(III), ... (4 entities in total)
• Functional Keywords: tandem ga base pairs, ga mismatch, dna
• Total number of polymer chains: 2
• Total formula weight: 6397.81

Authors

Gao, Y.-G.,Robinson, H.,Sanishvili, R.,Joachimiak, A.,Wang, A.H.-J. (deposition date: 1999-10-26, release date: 1999-11-05, Last modification date: 2024-02-07)

Primary citation

Gao, Y.G.,Robinson, H.,Sanishvili, R.,Joachimiak, A.,Wang, A.H.
Structure and recognition of sheared tandem G x A base pairs associated with human centromere DNA sequence at atomic resolution.
Biochemistry, 38:16452-16460, 1999

PubMed Abstract: G x A mismatched base pairs are frequently found in nucleic acids. Human centromere DNA sequences contain unusual repeating motifs, e.g. , (GAATG)n x (CATTC)n found in the human chromosome. The purine-rich strand of this repeating pentamer sequence forms duplex and hairpin structures with unusual stability. The high stability of these structures is contributed by the "sheared" G x A base pairs which present a novel recognition surface for ligands and proteins. We have solved the crystal structure, by the multiple-wavelength anomalous diffraction (MAD) method of d(CCGAATGAGG) in which the centromere core sequence motif GAATG is embedded. Three crystal forms were refined to near-atomic resolution. The structures reveal the detailed conformation of tandem G x A base pairs whose unique hydrogen-bonding surface has interesting interactions with bases, hydrated magnesium ions, cobalt(III)hexaammine, spermine, and water molecules. The results are relevant in understanding the structure associated with human centromere sequence in particular and G x A base pairs in nucleic acids (including RNA, like ribozyme) in general.

PubMed: 10600106
DOI: 10.1021/bi9914614

Experimental method

X-RAY DIFFRACTION (1.2 Å)