1D32

DRUG-INDUCED DNA REPAIR: X-RAY STRUCTURE OF A DNA-DITERCALINIUM COMPLEX

Summary for 1D32

• Entry DOI: 10.2210/pdb1d32/pdb
• Descriptor: DNA (5'-D(*CP*GP*CP*G)-3'), DITERCALINIUM (3 entities in total)
• Functional Keywords: right handed dna, double helix, complexed with drug, dna
• Total number of polymer chains: 2
• Total formula weight: 3102.56

Authors

Gao, Q.,Williams, L.D.,Egli, M.,Rabinovich, D.,Chen, S.-L.,Quigley, G.J.,Rich, A. (deposition date: 1991-01-23, release date: 1992-04-15, Last modification date: 2024-02-07)

Primary citation

Gao, Q.,Williams, L.D.,Egli, M.,Rabinovich, D.,Chen, S.L.,Quigley, G.J.,Rich, A.
Drug-induced DNA repair: X-ray structure of a DNA-ditercalinium complex.
Proc.Natl.Acad.Sci.USA, 88:2422-2426, 1991

PubMed Abstract: Ditercalinium is a synthetic anticancer drug that binds to DNA by bis-intercalation and activates DNA repair processes. In prokaryotes, noncovalent DNA-ditercalinium complexes are incorrectly recognized by the uvrABC repair system as covalent lesions on DNA. In eukaryotes, mitochondrial DNA is degraded by excess and futile DNA repair. Using x-ray crystallography, we have determined, to 1.7 A resolution, the three-dimensional structure of a complex of ditercalinium bound to the double-stranded DNA fragment [d(CGCG)]2. The DNA in the complex with ditercalinium is kinked (by 15 degrees) and severely unwound (by 36 degrees) with exceptionally wide major and minor grooves. Recognition of the DNA-ditercalinium complex by uvrABC in prokaryotes, and by mitochondrial DNA repair systems in eukaryotes, might be related to drug-induced distortion of the DNA helix.

PubMed: 2006181
DOI: 10.1073/pnas.88.6.2422

Experimental method

X-RAY DIFFRACTION (1.7 Å)