1D2Z

THREE-DIMENSIONAL STRUCTURE OF A COMPLEX BETWEEN THE DEATH DOMAINS OF PELLE AND TUBE

Summary for 1D2Z

• Entry DOI: 10.2210/pdb1d2z/pdb
• Descriptor: DEATH DOMAIN OF PELLE, DEATH DOMAIN OF TUBE, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (4 entities in total)
• Functional Keywords: six-helix bundle, linear array of death domains, plastic interfaces, apoptosis
• Biological source: Drosophila melanogaster (fruit fly); More
• Cellular location: Cell membrane: Q05652; Cytoplasm: P22812
• Total number of polymer chains: 4
• Total formula weight: 59300.01

Authors

Xiao, T.,Towb, P.,Wasserman, S.A.,Sprang, S.R. (deposition date: 1999-09-28, release date: 1999-11-29, Last modification date: 2024-02-07)

Primary citation

Xiao, T.,Towb, P.,Wasserman, S.A.,Sprang, S.R.
Three-dimensional structure of a complex between the death domains of Pelle and Tube.
Cell(Cambridge,Mass.), 99:545-555, 1999

PubMed Abstract: The interaction of the serine/threonine kinase Pelle and adaptor protein Tube through their N-terminal death domains leads to the nuclear translocation of the transcription factor Dorsal and activation of zygotic patterning genes during Drosophila embryogenesis. Crystal structure of the Pelle and Tube death domain heterodimer reveals that the two death domains adopt a six-helix bundle fold and are arranged in an open-ended linear array with plastic interfaces mediating their interactions. The Tube death domain has an insertion between helices 2 and 3, and a C-terminal tail making significant and indispensable contacts in the heterodimer. In vivo assays of Pelle and Tube mutants confirmed that the integrity of the major heterodimer interface is critical to the activity of these molecules.

PubMed: 10589682
DOI: 10.1016/S0092-8674(00)81542-1

Experimental method

X-RAY DIFFRACTION (2 Å)