1CQ4
CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59
Summary for 1CQ4
| Entry DOI | 10.2210/pdb1cq4/pdb |
| Descriptor | PROTEIN (SERINE PROTEINASE INHIBITOR 2), SULFATE ION, ... (4 entities in total) |
| Functional Keywords | serine protease inhibitor, polyglutamine insertion mutant, subtilisin- chymotrypsin inhibitor-2, immune system, hydrolase inhibitor |
| Biological source | Hordeum vulgare More |
| Total number of polymer chains | 2 |
| Total formula weight | 8185.52 |
| Authors | Chen, Y.W.,Stott, K.R. (deposition date: 1998-11-17, release date: 1998-11-25, Last modification date: 2023-08-09) |
| Primary citation | Chen, Y.W.,Stott, K.,Perutz, M.F. Crystal structure of a dimeric chymotrypsin inhibitor 2 mutant containing an inserted glutamine repeat. Proc.Natl.Acad.Sci.USA, 96:1257-1261, 1999 Cited by PubMed Abstract: We have constructed mutants of chymotrypsin inhibitor 2 with short glutamine repeats inserted into its inhibitory loop. These mutants oligomerize when expressed in Escherichia coli. The dimer of a mutant with four glutamines now has been crystallized, and its structure has been solved by molecular replacement by using the wild-type monomer as a search model. The structure of each half of the dimer is found to be the same as that of the wild-type monomer, except around the glutamine insertion. It was proposed that the components of the oligomers are held together by hydrogen bonds between the main-chain and side-chain amides of the glutamine repeats. Instead, they appear to form by swapping domains on folding in E. coli, and the glutamine repeats connecting the components of the dimers are disordered. PubMed: 9990011DOI: 10.1073/pnas.96.4.1257 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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