1COO

THE COOH-TERMINAL DOMAIN OF RNA POLYMERASE ALPHA SUBUNIT

Summary for 1COO

• Entry DOI: 10.2210/pdb1coo/pdb
• Descriptor: RNA POLYMERASE ALPHA SUBUNIT (1 entity in total)
• Functional Keywords: transcription regulation, nucleotidyl transferase
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 11094.72

Authors

Jeon, Y.H.,Negishi, T.,Shirakawa, M.,Yamazaki, T.,Fujita, N.,Ishihama, A.,Kyogoku, Y. (deposition date: 1995-10-09, release date: 1996-03-08, Last modification date: 2024-04-10)

Primary citation

Jeon, Y.H.,Negishi, T.,Shirakawa, M.,Yamazaki, T.,Fujita, N.,Ishihama, A.,Kyogoku, Y.
Solution structure of the activator contact domain of the RNA polymerase alpha subunit.
Science, 270:1495-1497, 1995

PubMed Abstract: The structure of the carboxyl-terminal domain of the Escherichia coli RNA polymerase alpha subunit (alpha CTD), which is regarded as the contact site for transcription activator proteins and for the promoter UP element, was determined by nuclear magnetic resonance spectroscopy. Its compact structure of four helices and two long arms enclosing its hydrophobic core shows a folding topology distinct from those of other DNA-binding proteins. The UP element binding site was found on the surface comprising helix 1, the amino-terminal end of helix 4, and the preceding loop. Mutation experiments indicated that the contact sites for transcription activator proteins are also on the same surface.

PubMed: 7491496

Experimental method

SOLUTION NMR