1CLD
DNA-binding protein
Summary for 1CLD
| Entry DOI | 10.2210/pdb1cld/pdb |
| Descriptor | CD2-LAC9, CADMIUM ION (2 entities in total) |
| Functional Keywords | zinc-binding domain, transcription regulation |
| Biological source | Kluyveromyces lactis |
| Cellular location | Nucleus: P08657 |
| Total number of polymer chains | 1 |
| Total formula weight | 7330.23 |
| Authors | Gardner, K.H.,Coleman, J.E. (deposition date: 1995-06-06, release date: 1995-09-15, Last modification date: 2024-05-22) |
| Primary citation | Gardner, K.H.,Anderson, S.F.,Coleman, J.E. Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding domain. Nat.Struct.Biol., 2:898-905, 1995 Cited by PubMed Abstract: The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding. PubMed: 7552715DOI: 10.1038/nsb1095-898 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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