1CJG
NMR STRUCTURE OF LAC REPRESSOR HP62-DNA COMPLEX
Summary for 1CJG
| Entry DOI | 10.2210/pdb1cjg/pdb |
| Descriptor | DNA (5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*TP*C)-3'), PROTEIN (LAC REPRESSOR) (2 entities in total) |
| Functional Keywords | transcription regulation, lac operon, lac repressor, headpiece, lac operator, transcription-dna complex, transcription/dna |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 4 |
| Total formula weight | 27140.24 |
| Authors | Spronk, C.A.E.M.,Bonvin, A.M.J.J.,Radha, P.K.,Melacini, G.,Boelens, R.,Kaptein, R. (deposition date: 1999-04-14, release date: 2000-01-01, Last modification date: 2023-12-27) |
| Primary citation | Spronk, C.A.,Bonvin, A.M.,Radha, P.K.,Melacini, G.,Boelens, R.,Kaptein, R. The solution structure of Lac repressor headpiece 62 complexed to a symmetrical lac operator. Structure Fold.Des., 7:1483-1492, 1999 Cited by PubMed Abstract: Lactose repressor protein (Lac) controls the expression of the lactose metabolic genes in Escherichia coli by binding to an operator sequence in the promoter of the lac operon. Binding of inducer molecules to the Lac core domain induces changes in tertiary structure that are propagated to the DNA-binding domain through the connecting hinge region, thereby reducing the affinity for the operator. Protein-protein and protein-DNA interactions involving the hinge region play a crucial role in the allosteric changes occurring upon induction, but have not, as yet, been analyzed in atomic detail. PubMed: 10647179DOI: 10.1016/S0969-2126(00)88339-2 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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