1CEH

STRUCTURE AND FUNCTION OF THE CATALYTIC SITE MUTANT ASP99ASN OF PHOSPHOLIPASE A2: ABSENCE OF CONSERVED STRUCTURAL WATER

1CEH の概要

• エントリーDOI: 10.2210/pdb1ceh/pdb
• 分子名称: PHOSPHOLIPASE A2, CALCIUM ION (3 entities in total)
• 機能のキーワード: hydrolase (carboxylic ester)
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted: P00593
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13849.60

構造登録者

Kumar, A.,Sekharudu, C.,Ramakrishnan, B.,Dupureur, C.M.,Zhu, H.,Tsai, M.-D.,Sundaralingam, M. (登録日: 1994-11-16, 公開日: 1995-02-07, 最終更新日: 2024-11-20)

主引用文献

Kumar, A.,Sekharudu, C.,Ramakrishnan, B.,Dupureur, C.M.,Zhu, H.,Tsai, M.D.,Sundaralingam, M.
Structure and function of the catalytic site mutant Asp 99 Asn of phospholipase A2: absence of the conserved structural water.
Protein Sci., 3:2082-2088, 1994

PubMed Abstract: To probe the role of the Asp-99 ... His-48 pair in phospholipase A2 (PLA2) catalysis, the X-ray structure and kinetic characterization of the mutant Asp-99-->Asn-99 (D99N) of bovine pancreatic PLA2 was undertaken. Crystals of D99N belong to the trigonal space group P3(1)21 and were isomorphous to the wild type (WT) (Noel JP et al., 1991, Biochemistry 30:11801-11811). The 1.9-A X-ray structure of the mutant showed that the carbonyl group of Asn-99 side chain is hydrogen bonded to His-48 in the same way as that of Asp-99 in the WT, thus retaining the tautomeric form of His-48 and the function of the enzyme. The NH2 group of Asn-99 points away from His-48. In contrast, in the D102N mutant of the protease enzyme trypsin, the NH2 group of Asn-102 is hydrogen bonded to His-57 resulting in the inactive tautomeric form and hence the loss of enzymatic activity. Although the geometry of the catalytic triad in the PLA2 mutant remains the same as in the WT, we were surprised that the conserved structural water, linking the catalytic site with the ammonium group of Ala-1 of the interfacial site, was ejected by the proximity of the NH2 group of Asn-99. The NH2 group now forms a direct hydrogen bond with the carbonyl group of Ala-1.

PubMed: 7703854

実験手法

X-RAY DIFFRACTION (1.9 Å)