1CBG

THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER (TRIFOLIUM REPENS L.), A FAMILY 1 GLYCOSYL-HYDROLASE

1CBG の概要

• エントリーDOI: 10.2210/pdb1cbg/pdb
• 分子名称: CYANOGENIC BETA-GLUCOSIDASE (2 entities in total)
• 機能のキーワード: cyanogenic beta-glucosidase, hydrolase (o-glycosyl)
• 由来する生物種: Trifolium repens (white clover)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56419.66

構造登録者

Barrett, T.E.,Suresh, C.G.,Tolley, S.P.,Hughes, M.A. (登録日: 1995-07-31, 公開日: 1995-10-15, 最終更新日: 2024-10-23)

主引用文献

Barrett, T.,Suresh, C.G.,Tolley, S.P.,Dodson, E.J.,Hughes, M.A.
The crystal structure of a cyanogenic beta-glucosidase from white clover, a family 1 glycosyl hydrolase.
Structure, 3:951-960, 1995

PubMed Abstract: beta-glucosidases occur in a variety of organisms and catalyze the hydrolysis of aryl and alkyl-beta-D-glucosides as well as glucosides with only a carbohydrate moiety (such as cellobiose). The cyanogenic beta-glucosidase from white clover (subsequently referred to as CBG) is responsible for the cleavage of cyanoglucosides. Both CBG and the cyanoglucosides occur within the plant cell wall where they are found in separate compartments and only come into contact when the leaf tissue experiences mechanical damage. This results in the eventual production of hydrogen cyanide which acts as a deterrent to grazing animals. beta-glucosidases have been assigned to particular glycosyl hydrolase families on the basis of sequence similarity; this classification has placed CBG in family 1 (there are a total of over 40 families) for which a three-dimensional structure has so far not been determined. This is the first report of the three-dimensional structure of a glycosyl hydrolase from family 1.

PubMed: 8535788
DOI: 10.1016/S0969-2126(01)00229-5

実験手法

X-RAY DIFFRACTION (2.15 Å)