1C7S

BETA-N-ACETYLHEXOSAMINIDASE MUTANT D539A COMPLEXED WITH DI-N-ACETYL-BETA-D-GLUCOSAMINE (CHITOBIASE)

Summary for 1C7S

Related1QBA 1QBB 1QBC 1C7T
DescriptorBETA-N-ACETYLHEXOSAMINIDASE, DI(N-ACETYL-D-GLUCOSAMINE), SULFATE ION, ... (4 entities in total)
Functional Keywordsglycosyl hydrolase, beta-n-acetylhexosaminidase, chitinolysis, a/b(tim)-barrel, site directed mutagenesis, substrate nucleophile stabilizer mutation, hydrolase
Biological sourceSerratia marcescens
Cellular locationPeriplasm Q54468
Total number of polymer chains1
Total molecular weight96749.58
Authors
Prag, G.,Papanikolau, Y.,Tavlas, G.,Vorgias, C.E.,Petratos, K.,Oppenheim, A.B. (deposition date: 2000-03-14, release date: 2000-09-20, Last modification date: 2017-10-04)
Primary citation
Prag, G.,Papanikolau, Y.,Tavlas, G.,Vorgias, C.E.,Petratos, K.,Oppenheim, A.B.
Structures of chitobiase mutants complexed with the substrate Di-N-acetyl-d-glucosamine: the catalytic role of the conserved acidic pair, aspartate 539 and glutamate 540.
J.Mol.Biol., 300:611-617, 2000
PubMed: 10884356 (PDB entries with the same primary citation)
DOI: 10.1006/jmbi.2000.3906
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.8 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.220130.4%3.8%0.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report