1C35
SOLUTION STRUCTURE OF A QUADRUPLEX FORMING DNA AND ITS INTERMIDIATE
Summary for 1C35
| Entry DOI | 10.2210/pdb1c35/pdb |
| Descriptor | DNA (5'-D(*GP*GP*TP*TP*GP*GP*TP*GP*TP*GP*GP*TP*TP*GP*G)-3'), POTASSIUM ION (2 entities in total) |
| Functional Keywords | aptamer, thrombin binding, potassium, metal binding sites, quadruplex, intermediate, dna |
| Total number of polymer chains | 1 |
| Total formula weight | 5525.01 |
| Authors | Bolton, P.H.,Marathias, V.M.,Wang, K. (deposition date: 1999-07-25, release date: 1999-08-18, Last modification date: 2024-05-22) |
| Primary citation | Marathias, V.M.,Bolton, P.H. Structures of the potassium-saturated, 2:1, and intermediate, 1:1, forms of a quadruplex DNA. Nucleic Acids Res., 28:1969-1977, 2000 Cited by PubMed Abstract: Potassium can stabilize the formation of chair- or edge-type quadruplex DNA structures and appears to be the only naturally occurring cation that can do so. As quadruplex DNAs may be important in the structure of telomere, centromere, triplet repeat and other DNAs, information about the details of the potassium-quadruplex DNA interactions are of interest. The structures of the 1:1 and the fully saturated, 2:1, potassium-DNA complexes of d(GGTTGGTGTGGTTGG) have been determined using the combination of experimental NMR results and restrained molecular dynamics simulations. The refined structures have been used to model the interactions at the potassium binding sites. Comparison of the 1:1 and 2:1 potassium:DNA structures indicates how potassium binding can determine the folding pattern of the DNA. In each binding site potassium interacts with the carbonyl oxygens of both the loop thymine residues and the guanine residues of the adjacent quartet. PubMed: 10756199DOI: 10.1093/nar/28.9.1969 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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