1BSO

12-BROMODODECANOIC ACID BINDS INSIDE THE CALYX OF BOVINE BETA-LACTOGLOBULIN

Summary for 1BSO

Entry DOI	10.2210/pdb1bso/pdb
Descriptor	PROTEIN (BOVINE BETA-LACTOGLOBULIN A), 12-BROMODODECANOIC ACID (3 entities in total)
Functional Keywords	beta-lactoglobulin, ligand binding, x-ray crystal structure, transport protein
Biological source	Bos taurus (cattle)
Total number of polymer chains	1
Total formula weight	18666.48
Authors	Qin, B.Y.,Creamer, L.K.,Baker, E.N.,Jameson, G.B. (deposition date: 1998-08-29, release date: 1999-09-02, Last modification date: 2024-10-30)
Primary citation	Qin, B.Y.,Creamer, L.K.,Baker, E.N.,Jameson, G.B. 12-Bromododecanoic acid binds inside the calyx of bovine beta-lactoglobulin. FEBS Lett., 438:272-278, 1998 Cited by PubMed Abstract: The X-ray structure of bovine beta-lactoglobulin with the ligand 12-bromododecanoic acid as a model for fatty acids has been determined at a resolution of 2.23 A in the trigonal lattice Z form. The ligand binds inside the calyx, resolving a long-standing controversy as to where fatty-acid like ligands bind. The carboxylate head group lies at the surface of the molecule, and the lid to the calyx is open at the pH of crystallization (pH 7.3), consistent with the conformation observed in ligand-free bovine beta-lactoglobulin in lattice Z at pH 7.1 and pH 8.2. PubMed: 9827560 DOI: 10.1016/S0014-5793(98)01199-5 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.23 Å)

Structure validation