1BO0

MONOCYTE CHEMOATTRACTANT PROTEIN-3, NMR, MINIMIZED AVERAGE STRUCTURE

Summary for 1BO0

• Entry DOI: 10.2210/pdb1bo0/pdb
• NMR Information: BMRB: 4177
• Descriptor: PROTEIN (MONOCYTE CHEMOATTRACTANT PROTEIN-3) (1 entity in total)
• Functional Keywords: chemotactic cytokine, signaling protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 8974.48

Authors

Kwon, D.,Lee, D.,Sykes, B.D.,Kim, K.-S. (deposition date: 1998-08-10, release date: 1999-10-10, Last modification date: 2024-10-30)

Primary citation

Kim, K.S.,Rajarathnam, K.,Clark-Lewis, I.,Sykes, B.D.
Structural characterization of a monomeric chemokine: monocyte chemoattractant protein-3.
FEBS Lett., 395:277-282, 1996

PubMed Abstract: 1H-NMR spectroscopy and analytical ultracentrifugation studies reveal that monocyte chemoattractant protein-3 (MCP-3) is a monomer. NMR solution structure shows that MCP-3 adopts an alphabeta fold similar to what is observed in structures of other known chemokines. However, MCP-3 is unique in that it does not show a propensity to form dimers. The closely related chemokines MCP-1 and MCP-2 show a monomer-dimer equilibrium in sedimentation equilibrium studies (approximately 0.2-2 mg/ml). As these proteins are present at nanomolar concentrations in vivo, the results suggest that they are monomeric at functional concentrations and that the monomer is the functionally significant form of MCP-1, MCP-2 and MCP-3.

PubMed: 8898111
DOI: 10.1016/0014-5793(96)01024-1

Experimental method

SOLUTION NMR