1BC8

STRUCTURES OF SAP-1 BOUND TO DNA SEQUENCES FROM THE E74 AND C-FOS PROMOTERS PROVIDE INSIGHTS INTO HOW ETS PROTEINS DISCRIMINATE BETWEEN RELATED DNA TARGETS

1BC8 の概要

• エントリーDOI: 10.2210/pdb1bc8/pdb
• 分子名称: DNA (5'-D(*TP*AP*CP*CP*GP*GP*AP*AP*GP*T)-3'), DNA (5'-D(*AP*AP*CP*TP*TP*CP*CP*GP*GP*T)-3'), PROTEIN (SAP-1 ETS DOMAIN), ... (5 entities in total)
• 機能のキーワード: ets domain, dna-binding domain, winged helix-turn-helix, dna-binding specificity, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P28324
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 17439.04

構造登録者

Mo, Y.,Vaessen, B.,Johnston, K.,Marmorstein, R. (登録日: 1998-05-05, 公開日: 1998-12-01, 最終更新日: 2024-02-07)

主引用文献

Mo, Y.,Vaessen, B.,Johnston, K.,Marmorstein, R.
Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins.
Mol.Cell, 2:201-212, 1998

PubMed Abstract: SAP-1 is a member of the Ets transcription factors and cooperates with SRF protein to activate transcription of the c-fos protooncogene. The crystal structures of the conserved ETS domain of SAP-1 bound to DNA sequences from the E74 and c-fos promoters reveal that a set of conserved residues contact a GGA core DNA sequence. Discrimination for sequences outside this core is mediated by DNA contacts from conserved and nonconserved protein residues and sequence-dependent DNA structural properties characteristic of A-form DNA structure. Comparison with the related PU.1/DNA and GABPalpha/beta/DNA complexes provides general insights into DNA discrimination between Ets proteins. Modeling studies of a SAP-1/SRF/DNA complex suggest that SRF may modulate SAP-1 binding to DNA by interacting with its ETS domain.

PubMed: 9734357
DOI: 10.1016/S1097-2765(00)80130-6

実験手法

X-RAY DIFFRACTION (1.93 Å)