1BB8
N-TERMINAL DNA BINDING DOMAIN FROM TN916 INTEGRASE, NMR, 25 STRUCTURES
Summary for 1BB8
| Entry DOI | 10.2210/pdb1bb8/pdb |
| Descriptor | INTEGRASE (1 entity in total) |
| Functional Keywords | integrase, dna binding, transposition |
| Biological source | Enterococcus faecalis |
| Total number of polymer chains | 1 |
| Total formula weight | 8462.62 |
| Authors | Clubb, R.T.,Connolly, K.M.,Wojciak, J.M. (deposition date: 1998-04-29, release date: 1998-11-25, Last modification date: 2024-05-22) |
| Primary citation | Connolly, K.M.,Wojciak, J.M.,Clubb, R.T. Site-specific DNA binding using a variation of the double stranded RNA binding motif. Nat.Struct.Biol., 5:546-550, 1998 Cited by PubMed Abstract: The integrase family of site-specific recombinases catalyze a diverse array of DNA rearrangements in archaebacteria, eubacteria and yeast. The solution structure of the DNA binding domain of the integrase protein from the conjugative transposon Tn916 has been determined using NMR spectroscopy. The structure provides the first insights into distal site DNA binding by a site-specific integrase and reveals that the N-terminal domain is structurally similar to the double stranded RNA binding domain (dsRBD). The results of chemical shift mapping experiments suggest that the integrase protein interacts with DNA using residues located on the face of its three stranded beta-sheet. This surface differs from the proposed RNA binding surface in dsRBDs, suggesting that different surfaces on the same protein fold can be used to bind DNA and RNA. PubMed: 9665166DOI: 10.1038/799 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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