1B9Q

NMR STRUCTURE OF HEPARIN BINDING SITE OF NON COLLAGENOUS DOMAIN I (NC1) OF COLLAGEN FACIT XIV

Summary for 1B9Q

• Entry DOI: 10.2210/pdb1b9q/pdb
• Descriptor: PROTEIN (COLLAGEN ALPHA 1) (1 entity in total)
• Functional Keywords: collagen facit xiv, heparin-binding site, nc1
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 4020.76

Authors

Montserret, R.,Deleage, G.,Penin, F. (deposition date: 1999-02-15, release date: 1999-02-25, Last modification date: 2023-12-27)

Primary citation

Montserret, R.,Aubert-Foucher, E.,McLeish, M.J.,Hill, J.M.,Ficheux, D.,Jaquinod, M.,van der Rest, M.,Deleage, G.,Penin, F.
Structural analysis of the heparin-binding site of the NC1 domain of collagen XIV by CD and NMR.
Biochemistry, 38:6479-6488, 1999

PubMed Abstract: Type XIV collagen, a fibril-associated collagen with interrupted triple helices (FACIT), interacts with the surrounding extracellular matrix and/or with cells via its binding to glycosaminoglycans (GAGs). To further characterize such interactions in the NC1 domain of chicken collagen XIV, we identified amino acids essential for heparin binding by affinity chromatography analysis after proteolytic digestion of the synthetic peptide NC1(84-116). The 3D structure of this peptide was then obtained using circular dichroism and NMR. The NC1(84-116) peptide appeared poorly structured in water, but the stabilization of its conformation by the interaction with hydrophobic surfaces or by using cosolvents (TFE, SDS) revealed a high propensity to adopt an alpha-helical folding. A 3D structure model of NC1(84-116), calculated from NMR data recorded in a TFE/water mixture, showed that the NC1-heparin binding site forms a amphipathic alpha-helix exhibiting a twisted basic groove. It is structurally similar to the consensus spatial alpha-helix model of heparin-binding [Margalit et al. (1993) J. Biol. Chem. 268, 19228-19231], except that the GAG binding domain of NC1 may be extended over 18 residues, that is, the NC1(94-111) segment. In addition, the formation of a hydrophobic groove upon helix formation suggests the contribution of additional sequences to ensure the stability of the GAG-binding domain. Overall the NC1(84-116) model exhibits a nativelike conformation which presents suitably oriented residues for the interaction with a specific GAG.

PubMed: 10350466
DOI: 10.1021/bi9900222

Experimental method

SOLUTION NMR