1B96

ANALYSIS OF A MUTATIONAL HOT-SPOT IN THE ECORV RESTRICTION ENDONUCLEASE: A CATALYTIC ROLE FOR A MAIN CHAIN CARBONYL GROUP

Summary for 1B96

DescriptorDNA (5'-D(*AP*AP*AP*GP*AP*TP*AP*TP*CP*TP*T)-3'), RESTRICTION ENDONUCLEASE ECORV (3 entities in total)
Functional Keywordsendonuclease, restriction, ecorv, hydrolase-dna complex, hydrolase/dna
Biological sourceEscherichia coli
Total number of polymer chains4
Total molecular weight63832.76
Authors
Thomas, M.P.,Halford, S.E.,Brady, R.L. (deposition date: 1999-02-19, release date: 1999-02-26, Last modification date: 2018-03-07)
Primary citation
Thomas, M.P.,Brady, R.L.,Halford, S.E.,Sessions, R.B.,Baldwin, G.S.
Structural analysis of a mutational hot-spot in the EcoRV restriction endonuclease: a catalytic role for a main chain carbonyl group.
Nucleic Acids Res., 27:3438-3445, 1999
PubMed: 10446231 (PDB entries with the same primary citation)
DOI: 10.1093/nar/27.17.3438
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.3 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRSRZ outliers120.4%8.0%3.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution