1B8H
SLIDING CLAMP, DNA POLYMERASE
Summary for 1B8H
| Entry DOI | 10.2210/pdb1b8h/pdb |
| Descriptor | DNA POLYMERASE PROCESSIVITY COMPONENT, DNA POLYMERASE fragment (2 entities in total) |
| Functional Keywords | sliding clamp, gp45, replisome, accessory protein, transferase |
| Biological source | Enterobacteria phage RB69 More |
| Total number of polymer chains | 4 |
| Total formula weight | 76751.01 |
| Authors | Shamoo, Y.,Steitz, T.A. (deposition date: 1999-02-01, release date: 1999-02-09, Last modification date: 2023-08-09) |
| Primary citation | Shamoo, Y.,Steitz, T.A. Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex. Cell(Cambridge,Mass.), 99:155-166, 1999 Cited by PubMed Abstract: We have solved the crystal structures of the bacteriophage RB69 sliding clamp, its complex with a peptide essential for DNA polymerase interactions, and the DNA polymerase complexed with primer-template DNA. The editing complex structure shows a partially melted duplex DNA exiting from the exonuclease domain at an unexpected angle and significant changes in the protein structure. The clamp complex shows the C-terminal 11 residues of polymerase bound in a hydrophobic pocket, and it allows docking of the editing and clamp structures together. The peptide binds to the sliding clamp at a position identical to that of a replication inhibitor peptide bound to PCNA, suggesting that the replication inhibitor protein p21CIP1 functions by competing with eukaryotic polymerases for the same binding pocket on the clamp. PubMed: 10535734DOI: 10.1016/S0092-8674(00)81647-5 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
Download full validation report
