1AU1

HUMAN INTERFERON-BETA CRYSTAL STRUCTURE

1AU1 の概要

• エントリーDOI: 10.2210/pdb1au1/pdb
• 分子名称: INTERFERON-BETA, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-[alpha-D-quinovopyranose-(1-6)]beta-D-glucopyranose, alpha-D-quinovopyranose-(1-6)-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: interferon, helical cytokine, immune system, cytokine
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P01574
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41636.87

構造登録者

Karpusas, M.,Nolte, M.,Lipscomb, W. (登録日: 1997-09-09, 公開日: 1998-06-17, 最終更新日: 2024-11-20)

主引用文献

Karpusas, M.,Nolte, M.,Benton, C.B.,Meier, W.,Lipscomb, W.N.,Goelz, S.
The crystal structure of human interferon beta at 2.2-A resolution.
Proc.Natl.Acad.Sci.USA, 94:11813-11818, 1997

PubMed Abstract: Type I interferons (IFNs) are helical cytokines that have diverse biological activities despite the fact that they appear to interact with the same receptor system. To achieve a better understanding of the structural basis for the different activities of alpha and beta IFNs, we have determined the crystal structure of glycosylated human IFN-beta at 2.2-A resolution by molecular replacement. The molecule adopts a fold similar to that of the previously determined structures of murine IFN-beta and human IFN-alpha2b but displays several distinct structural features. Like human IFN-alpha2b, human IFN-beta contains a zinc-binding site at the interface of the two molecules in the asymmetric unit, raising the question of functional relevance for IFN-beta dimers. However, unlike the human IFN-alpha2b dimer, in which homologous surfaces form the interface, human IFN-beta dimerizes with contact surfaces from opposite sides of the molecule. The relevance of the structure to the effects of point mutations in IFN-beta at specific exposed residues is discussed. A potential role of ligand-ligand interactions in the conformational assembly of IFN receptor components is discussed.

PubMed: 9342320
DOI: 10.1073/pnas.94.22.11813

実験手法

X-RAY DIFFRACTION (2.2 Å)