1ARO

T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME

1ARO の概要

• エントリーDOI: 10.2210/pdb1aro/pdb
• 分子名称: T7 RNA POLYMERASE, T7 LYSOZYME, MERCURY (II) ION (3 entities in total)
• 機能のキーワード: transcription, dna-directed rna polymerase, hydrolase, glycosidase, complex (polymerase-hydrolase), complex (polymerase-hydrolase) complex, complex (polymerase/hydrolase)
• 由来する生物種: Enterobacteria phage T7; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 117347.48

構造登録者

Steitz, T.,Jeruzalmi, D. (登録日: 1997-08-08, 公開日: 1998-10-21, 最終更新日: 2024-02-07)

主引用文献

Jeruzalmi, D.,Steitz, T.A.
Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme.
EMBO J., 17:4101-4113, 1998

PubMed Abstract: The T7 RNA polymerase-T7 lysozyme complex regulates phage gene expression during infection of Escherichia coli. The 2.8 A crystal structure of the complex reveals that lysozyme binds at a site remote from the polymerase active site, suggesting an indirect mechanism of inhibition. Comparison of the T7 RNA polymerase structure with that of the homologous pol I family of DNA polymerases reveals identities in the catalytic site but also differences specific to RNA polymerase function. The structure of T7 RNA polymerase presented here differs significantly from a previously published structure. Sequence similarities between phage RNA polymerases and those from mitochondria and chloroplasts, when interpreted in the context of our revised model of T7 RNA polymerase, suggest a conserved fold.

PubMed: 9670025
DOI: 10.1093/emboj/17.14.4101

実験手法

X-RAY DIFFRACTION (2.8 Å)